Effect of non‑surgical periodontal therapy on CD14 + CD16+ monocyte counts in peripheral blood samples: a clinical interventional study

Abstract

Monocytes and their macrophage progeny are thought to be involved in tissue and alveolar bone destruction in periodontal disease. It has been documented that the proportion of (CD14 + CD16+) non-classical monocytesin the blood are elevated in chronic periodontitis;A total of 20 chronic generalized periodontitis patients who wereotherwise healthy, were recruited for this study. At baseline and 3 weeks after non-surgical periodontal treatment,peripheral blood was obtained to assess the levels of C-reactive protein (CRP) and the proportion of monocytesubsets. Monocyte subsets were assessed using flow cytometry;The mean percentage of CD14 + CD16+ non-classicalmonocytes in the peripheral blood sample at baseline was 13.95 + 2.09, that reduced to 8.94 + 1.23 3 weeks after nonsurgicaltreatment. A distinct significant reduction in the percentage of non-classical monocytes and a concomitantincrease in classical monocytes were observed following periodontal treatment compared to baseline. Therewas a significant reduction in the all the periodontal parameters and CRP levels 3 weeks post non-surgical periodontaltreatment. A positive correlation between CRP and percentage of non-classical monocytes was also observed; Periodontaltreatment potentially modulates the host response effectively